Zentrosomako TEDC2 proteinaren potentzial tumorigenikoa aztertzeko sagu transgenikoaren garapena

Abstract

The centrosome is an organelle of animal cells that nucleates microtubules and whose defects are associated with cancer initiation and development. The centrosomal protein TEDC2 has a high tumorigenic potential, as it is overexpressed in most tumor samples and is associated with a worse prognosis of cancer patients. To investigate the role of TEDC2 overexpression in cancer, in this work we have generated a transgenic mouse model in which the overexpression of this protein can be induced by the Tet-On system. We have confirmed that the transgenes required for the functioning of the system are integrated into the mouse genome and that, upon doxycycline addition, we are able to increase Tedc2 mRNA levels in fibroblast cell lines derived from these mice.