Fluxu-zitometria bidez sagu-laginak immunofenotipatzeko lan-fluxuaren diseinua

Abstract

Recent studies, along with the described E2f2-/- mice phenotype, suggest that E2F transcription factors play a key role in the regulation of the immune response. Nevertheless, the immune mechanism responsible for the phenotype of E2f2-/- mice remains elusive. Immunophenotyping by flow cytometry allows for the identification of cells implicated in pathological processes and therapeutic responses. However, no standardized workflow is designed to immunophenotype mice-derived samples. In the present work, we developed a strategy for characterizing immune cell populations from different mouse organs and tissues to establish a reliable approach for their analysis. The designed strategy allows for the study of the immune response in E2f2-/- mice in different experimental conditions.