Nanoegiturazko biomaterial magnetikoen aplikazioa ehun-ingeniaritzan

Abstract

The possibility to control remoting magnetically on connective tissue cells has opened up new possibilities in regenerative medicine. Cells that internalise magnetic nanoparticles can be manipulated by external magnetic fields. The action of these fields stimulates mechanosensitive channels, which trigger a biological response that affects proliferation, adhesion and intercellular interaction in tissue formation. The aim of this thesis is to validate the development of new biocompatible nanomaterials that allow effective control of magnetic field-assisted tissue regeneration. To this end, disc-shaped nanostructures will be fabricated with two important distinctive features compared to the nanoparticles that have been used to date: their magnetic moment is much higher and their shape asymmetry will allow torques to be applied as a source of cell stimulation. The efficacy of these biomaterials will be verified in two-dimensional cultures of endothelial cells and fibroblasts on substrates with magnetic island patterns and in three-dimensional cultures with the formation of spheroids.