Gertuko biotinilazioa E2F7 transkripzio faktorearen interaktoma deskribatzeko estrategia gisa: TurboID sistemaren garapena

Authors

  • Ekaitz Madariaga University of the Basque Country (UPV/EHU)
  • James D. Sutherland CIC bioGUNE
  • Jone Mitxelena University of the Basque Country (UPV/EHU)
  • Ana M. Zubiaga University of the Basque Country (UPV/EHU)

DOI:

https://doi.org/10.26876/ikergazte.v.04.15

Keywords:

E2F7, interactome, proximity biotinylation, TurboID

Abstract

E2F transcription factors (E2F1-8) play an important role in several biological processes and diseases. Regarding E2F7, we have shown that its main role is to repress expression of protein- and microRNA-coding genes, but little is known about its mechanism. In this work, we describe the development of a proximity biotinylation approach based on the TurboID biotin ligase in order to study E2F7 interactors. With this aim, we generated stable cell lines that express TurboID-E2F7 in response to doxycycline. Our data confirm that TurboID-E2F7 localizes mainly in the nucleus, similarly to the wild-type E2F7, and describe the presence of E2F7 interactors in both the nucleus and the cytoplasm. In addition, we show that the TurboID strategy is suitable for identification of E2F7 interactors.

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Copyright (c) 2023 IkerGazte. Nazioarteko ikerketa euskaraz

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

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Published

2023-05-09

How to Cite

Madariaga, E., Sutherland, J. D., Mitxelena, J., & Zubiaga, A. M. (2023). Gertuko biotinilazioa E2F7 transkripzio faktorearen interaktoma deskribatzeko estrategia gisa: TurboID sistemaren garapena. IkerGazte. Nazioarteko Ikerketa Euskaraz, 4, 123–130. https://doi.org/10.26876/ikergazte.v.04.15

Conference Proceedings Volume

Vol. 4 (2023): V. Ikergazte. Nazioarteko ikerketa euskaraz. Kongresuko artikulu bilduma. Osasun Zientziak

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Artikuluak

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